earticle

영어

Carbohydrate microarray might have powerful potential for assaying substrate specificity or activity of carbohydrate-processing enzymes such as glycosyltransferase and glycosidase. However, carbohydrate microarray system has been rarely utilized because complex chemical reactions are demanded for microarray preparation. Recently, we have been developed a simple and cost-effective method for fabrication of carbohydrate microarray which uses the reaction of amine-conjugated carbohydrates with glass surface. In the present work, we employed the developed carbohydrate microarray system to rapidly determine substrate specificity or activity of glycosyltransferase. Pasteurella multocidaoriginated sialyltransferase was used as a target glycosyltransferase because it is a wonderful target to prove the potential of carbohydrate microarray. Collectively, the functional carbohydrate microarray would be a powerful analytical tool to screen substrate specificity or other properties of carbohydrate-processing enzymes.