원문정보
초록
영어
Colorectal cancer is the third leading cause of cancer-related death in the Korea. It is eager to find a method for preventing and curing colorectal cancer. The tumor-associated antigen GA733 is a cell surface glycoprotein highly expressed in colorectal carcinoma. In this study, GA733 was fused to Fc fragment of human IgG as a vaccine candidate in plant expression system. The fusion protein GA733-Fc can improve their expression in plant cells and induce cellular and humoral immune responses. GA733-Fc is glycosylated, however the N-glycan structures of plant-derived GA733-Fc differ from their parental counterparts. Plant-derived specific N-glycoforms have the potential to induce immunogenicity and allergenic responses in human therapy. Thus, oligomannose glycan structure was tried to be generated through retaining GA733-Fc and GA733 in ER with KDEL sequence. Agrobacterium-mediated transformation was conducted to generate transgenic plant expressing GA733-Fc and GA733. Recombinant protein expression, accumulation and biological activity of GA733-Fc with KDEL (GA733-FcK), GA733-Fc without KDEL (GA733-Fc), and GA733 with KDEL (GA733K) in transgenic plants were compared by western blot, confocal immunofluorescence analysis, ELISA and mouse immunization, respectively.