원문정보
초록
영어
Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we obtained low-molecular weight fuco-oligosaccharides (LMFOs), ranging from 1,389 to 3,749 Da from the fucoidan of Korean Undaria pinnatifida sporophyll by crude enzyme preparation of Sphingomonas paucimobilis PF-1 (KCTC 11130BP). The fucoidan and LMFOs had potent antiviral activity against herpes simplex virus types 1 (HSV-1) any detectable level of cytotoxicity on Vero cells. Fucoidan and LMFOs contained strong anti-HSV activity with 50% inhibitory (IC50) at 2.43 and 2.65 μg/ml concentration. Interestingly, they also showed inhibitory activity directly against viral particles as observed in a virucidal assay (88.4%, and 70.5% at 5 μg/ml concentration). Respectively, LMFOs exerted anti-HSV activity when pre-incubated with host cells prior to viral infection, suggesting that a main inhibitory could be mediated via prevention of viral entry. The antiviral activity was significantly affected depending on the degree of sulfation ; antiviral activity was significantly reduced upon removal of sulfate groups. From the FT-IR and NMR analysis, sulfate groups were shown to be located at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-HSV activity of this polymer.