earticle

영어

Glycosylation of small molecule based therapeutics and natural products influences biological activities by altering molecular and cellular specificities. E. coli has been engineered by expressing unnatural TDP-4-amino-4, 6-dideoxy-D-galactose biosynthetic pathway genes to produce pools of activated TDP-aminodeoxysugar, and glycosyltransferase gene from A. thaliana. Quercetin and Kaempferol Deoxyaminosugar conjugates were produced by whole cell biocatalysis and the products were analysed by TLC and Proton NMR. This strategy of in vivo glycosylation offers vast combinatorial biosynthesis potential to produce glycorandomized natural products of pharmaceutical importance by simple fermentation.