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Silver nanoparticles (AgNPs) are nanoparticles of silver that between 1 nm and 100 nm in size and are prepared by reduction of Ag+ ions to Ag metal by chemical reducing agents as a common method. It has been reported that the bactericidal effects of AgNPs are dependent on size with preferential size of ~1-10 nm. In recent days biological molecules instead of chemical reducing agents have been reported to synthesize AgNPs including plant extracts, polysaccharide, fungi, DNA, yeast, and bacteria. Recently, research articles report the use of polysaccharides for the synthesis of AgNPs. Among polysaccharides, glycosaminoglycans (GAGs: Chondroitin sulfate (CS) and Acharan sulfate (AS)) exhibit diverse biological activities through interactions between physiological proteins. Owing to biological activities of GAGs, we could expect synergistic effects of GAGs-derived nanomaterials from GAGs and nanoparticles. AgNPs-GAGs represented several activities such as anti-oxidant, anti- bacterial, and wound healing. AgNPs by using the CS exhibited antioxidative, where as CS only remained almost inactive. AgNPs has no cytotoxicity on fibroblast cells. When cells were treated with AgNPs by using GAGs for 24 hr, there were no cytotoxicity effects on fibroblast cells. AgNPs-GAGs displayed significant inhibitory action against both antibiotic-susceptible and resistant strains of many strains with MIC ranging from 1 to 8 mg/ml. AgNPs-CS showed the highest inhibitory activity against Enterobacter cloacae 1321E with MICs of 1 mg/ml. At animal model, after excision skin, wound size of AgNPs-CS and AgNPs-AS ointment group was smaller than not treated and Vaseline group. Time to closure was defined as the time at which the wound bed was completely filled in with new tissue. Therefore, the utilization of such biological entities and environmentally benign solvents, and renewable materials are some of the key issues that merit important consideration in a green synthetic strategy.