원문정보
초록
영어
O-GlcNAcylation is a dynamic posttranslational modification which controls many nuclear and cytosolic protein activity. It features attachment and removal of O-linked β-N-acetylglucosamine on ser/thr residues. Cycling of O-GlcNAc is known to be regulated by two enzymes, O-GlcNAc transferase (OGT) involved in addition of O-GlcNAc and O-GlcNAcase (OGA) which catalases removal of O-GlcNAc. O-GlcNAc modification is concerned with many cellular events such as transcription, proteasomal degradation, apoptosis, proliferation and cell cycle, however, there is a limitation to produce a large amount of O-GlcNAc modified proteins in mammalian cells. So in this study, we make an attempt to establish E.coli plant which enables us to get much O-GlcNAcylated targets in more efficient way. Expected impact of this research is making it easy to generate site-specific antibody, which will lead to gather momentum to study various functions of this modification on target proteins.