earticle

영어

The anti-complementary substance is able to prevent hemolytic activity by the activation or inhibition of the complement system. Some anti-complementary polysaccharides have been isolated from bacteria, fungi, and plants. In order to develope new mushrooms available as functional food with excellent immuno-modulating activity, we investigated the anti-complementary activities of water soluble polysaccharides extracted from fruiting bodies of 5 mushrooms which have known as traditional medicinal mushrooms for a long time. It was observed that the crude polysaccharides of JFMF mushroom had a potent anti-complementary activity. Crude polysaccharide of JFMF was isolated into a neutral polysaccharide (JFMF-NP) and three acidic polysaccharides (JFMF-AP1, AP2, AP3) by DEAE-sepharose column chromatography. JFMF-AP2, one of these, showed the very excellent anti-complementary activity of ITCH (inhibition of total complement hemolysis) value 70% on 5 ug/ml concentrations. Its molecular weight was estimated less about 5,000 by Toyopearl HW-65F gel filtration chromatography using pullulan molecular size standard kits. Also, detection of a cleavage of C3 by immunoelectrophoresis indicated that JFMF-NP and JFMF-AP2 activates the complement via both the alternative and classical pathways.