원문정보
초록
영어
Understanding of the molecular relationships in carbohydrate-protein interactions provides useful information on biological processes in living organisms and is also helpful for development of potent biomedical agents. Herein, the interaction unbinding force between GM1 pentasaccharide and Vibrio cholera toxin (ctx) proteins was measured by using atomic force microscopy (AFM), which enabled us to determine the interaction of ctx holotoxin (ctxAB) with GM1 and the interactive From the analogue analyses, we understood how structural and binding positional differences in complex carbohydrate affect the interaction with protein and surmise that the GM1-ctxAB complex makes a “two-finger grip” formation through the conformational change of a flexible carbohydrate. Inconclusion, using AFM force analysis, we successfully quantified and characterized the interactive configuration of carbohydrate-protein molecules.