원문정보
초록
영어
Autoantibodies, which are generated by immune system recognizing the presence of the abnormal tumor-associated antigens, are promising biomarkers for the early detection of tumors. Recently, we established hundreds of B cell hybridomas from H-ras12V transgenic mouse, a typical tumor model system, as a source of tumor-associated autoantibodies without using any extracellular antigens and have characterized specific target antigens against them. TAB-K94 monoclonal antibody, one of tumor-associated autoantibodies obtained from H-ras12V transgenic mouse, was investigated in this study and its target antigen was identified as cytokeratin 8/18 (CK8/18) complex, an intermediate filament (IF) protein complex of epithelial cells which is involved in cell motility and cancer progression. To establish a method for the detection of autoantibodies against CK8/18 complex in tumor patients sera, a specific mimotope against TAB-K94 autoantibody was screened from the cyclic random hepta-peptide phage library and, by enzyme-linked immunosorbent assay (ELISA) using it as a binder of anti-CK8/18 autoantibodies, we could distinguish breast cancer patients vs. normal subjects with 50.00 % sensitivity and 82.61 % specificity. These results imply that anti-CK8/18 autoantibody is induced in a certain group of breast cancer patients and detection of anti-CK8/18 autoantibody would be useful for the diagnosis of breast cancer.