earticle

영어

Dynamic protein modification, called post-translational modification (PTM), is the chemical modification of a protein and an important biological regulatory mechanism to understand cellular function and interactions. Glycosylation is one of the most common post-translational modifications of proteins and is involved in many physiological functions and biological pathways. Analysis of glycoproteins including site mapping and glycan characterization is an essential sub-field of proteomics using high throughput techniques by mass spectrometry. Recent improvements in mass spectrometry provide sensitive methodologies by liquid chromatography tandem mass spectrometry (LC-MS/MS) as an innovative analytical technology applicable to a wide number of analyses in glycoproteins. In addition to mapping sites of N-linked glycosylation, mapping sites of O-linked glycosylation including are another current challenge in proteomics. Here we demonstrate that the methodologies allow for analyses of glycoproteins and their mapping sites in various biological species and also discuss the effective uses of mass spectrometry for glycomic analysis and modern techniques of biomolecular mass spectrometry with the glycome from glycoproteins.