earticle

영어

Gangliosides are complex glycosphingolipids that contain one or more sialic acids, the major components of cytoplasmic cell membranes. These are thought to play a role in the control of biological process including cell surface interaction, cell differentiation and transmemebrane signaling. In this study, we demonstrated that change of gangliosides pattern and glycosyltransferase gene in mouse induced pluripotent stem cells (miPSCs). The miPSCs were derived from OG2 mouse embryonic fibroblasts (mEFs) using retrovirus-mediated delivery and expression of Oct-4, Sox-2, Klf-4 and c-Myc. These cells were expressed alkaline phosphatase (AP), stage specific embryonic antigen-1 (SSEA-1) and mRNA such as Oct-4, Sox-2 and Nanog. In HPTLC and immunochemistry analysis, ganglioside GM3 and GD1a were expressed in mEFs, miPSCs and mESCs. However, only GM2 and GM1 were expressed in mEFs and mESCs. Ganglioside GM3 and GD3 expression level was low in miPSCs. Moreover, A2B5 antigen, c-series gangliosides such as GT3 and GQ1c, was only expressed in mESCs. To provide a better understanding of the metabolic basis of glycosyltransferase genes expressions, we analyzed expression of glycosyltransferase genes in mEFs, miPSCs and mESCs, and found that expression of sialyltransferase-I (ST-I), ST-II, ST-III, N-acetylgalactosamine transferase (GalNAcT), galactosyltransferase-II (Gal-II), ST-IV and ST-V. However, ST-II was not only expressed in mEFs. These results suggest that ganglioside GM3 and GD1a unique and powerful cell-surface biomarker to identify of mESCs and miPSCs, and change of ganglioside expression could be affected by gangliosides synthase activation. Therefore, gangliosides may play a role in the cell differentiation process.