earticle

영어

Gangliosides are ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, regulatory roles of human leukocyte in the ganglioside expression of the primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To study whether human leukocyte are related to expressional changes of gangliosides in PAECs we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with fetal bovine serum (FBS), as control, or with recombinant human tumor necrosis factor-α (TNF-α), or with human serum and FBS contained with human leukocyte. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS are contained GM3, GM1 and GD3 as major gangliosides. However, ganglioside GM1 significantly decreased in PAECs incubated for 5hrs with TNF-α (10 ng/ml), or with 10% human serum and FBS contained with human leukocytes. Taken together, these results suggest that human TNF-α secreted by human leukocyte against PAECs and possibly happened expressional pattern changes of ganglioside GM1 in PAECs by secreted TNF-α. The finding that human leukocyte promotes expressional pattern changes of ganglioside in PAECs depending on TNF-α secreation may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.