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Poster-29

Expression of Human MAC-2 Binding Protein in Drosophila S2 Cells and Analysis of N-glycans Using HPLC & MALDI-MS

원문정보

Kyoung-Ro Kim, Dong-Gyun Kang, Young-Hwan Kim, Hyung-Joon Cha

한국당과학회 한국당과학회 학술대회 2010년 한국당과학회 하계학술대회 2010.06 pp.102-102
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Cancer that is one of serious diseases for human causes angiogenesis, metastasis, and abnormal cell divisions including genetic change. Thus, lots of researchers have been investigated to inhibit cancer. Human MAC-2 binding protein (or human 90K, h90K) has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having 7 N-glycosylation sites. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stably-transfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot analyses with hexa histidine tag and bioactivity check. And we compared with Chinese Hamster Ovary (CHO) cell-deived human MAC-2 binding protein for expression and activity check. We also identified glycosylation patterns of recombinant h90K glycoprotein using HPLC and MALDI-MS. This results and application technology will contribute for scientific and medical engineering.

저자정보

  • Kyoung-Ro Kim National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
  • Dong-Gyun Kang National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
  • Young-Hwan Kim Mass Spectrometry Research Team, Korea Basic Science Institute (KBSI)
  • Hyung-Joon Cha National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)

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자료제공 : 네이버학술정보

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