earticle

영어

Baculovirus expression system is considered to have several benefits for production of recombinant protein such as high capacity, flexibility, and similar human glycosylation pattern. Typically, before any large scale expression work was commenced, an accurate titering of recombinant baculovirus is required to ensure the most appropriate expression conditions. One of them, the method have been developed by BaculoELISA Titer Kit (Clontech, Mountain Biew, CA, USA). Stocks of any Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) type baculovirus can be titered using this kit. However, control virus of this kit is high cost and relatively small quantity in kit. In this study, production of mAb CO17-1A by baculovirus infection was performed at different times, concentrations and passages. Immunoblot analysis confirmed expression of mAb CO17-1A in the infected cells. mAb CO17-1A expressed significantly high level under the condition of baculovirus passages 3, 72h and 400㎕ in 5ml insect cells after infection. The results indicated that it is not necessary to use BaculoELISA assay.