원문정보
초록
영어
Colorectal cancer is the third leading cause of cancer-related death with 655,000 deaths worldwide per year in the Western world. The tumor-associated antigen GA733 is a glycoprotein highly expressed on colorectal carcinoma cell surface. We have fused GA733 to Fc fragment of human IgG as a vaccine candidate in plant expression system. The fusion protein GA733-Fc can improve their expression in plant cells and induce cellular and humoral host immune responses. GA733-Fc is glycosylated, but the N-glycanstructures of plant-derived GA733-Fc differ from their parental counterparts. Plant-derived specific N-glycoforms can potential to induce immunogenicity and allergenic responses in human therapy. Thus, we have tried to generate oligomannose glycan structure by retaining GA733-Fc and GA733 in ER with KDEL sequence. Agrobacterium-mediated transformation was conducted to generate transgenic plant expressing GA733-Fc and GA733. We have compared protein expression, accumulation and biological activity of GA733-Fc with KDEL, GA733-Fc without KDEL, GA733 with KDEL and GA733 without KDEL in transgenic plants by western blot, confocal immunofluorescence analysis, ELISA and mouse immunization, respectively.