원문정보
초록
영어
The by-products of marine organisms including salmon, skate, flatfish, and yellow goosefish were investigated to search for new source of chondroitin sulfate. Agarose gel electrophoresis with chondroitinase depolymerization showed that purified chondroitin sulfate did not contain any other glycosaminoglycans. 1H-NMR spectra were acquired to confirm the structure and purity. The average molecular weight ranging from 22 to 64 kDa was determined by high-performance size exclusion chromatography. Disaccharide compositions and purities were determined by strong anion exchange-high performance liquid chromatography after chondroitinase ABC depolymerization. Strong anion exchangehigh- performance size exclusion chromatography data exhibited that the purity was from 81.7±1.3 to 114.2±2.5% and the yield was from 1.3 to 12.5%. All analytical results indicate that salmon cartilage, skate cartilage, and yellow goosefish bone could be promising sources of chondroitin sulfate to substitute shark cartilage chondroitin sulfate in commercial neutraceuticals.