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Poster-11

Enzymatic transformation of platycosides and one-step separation of platycodin D by high-speed counter-current chromatography

초록

영어

Platycosides, the saponins found in the roots of Platycodon grandiflorum (PlatycodiRadix), are typically composed of oleanene backbones with two side chains. Of the platycosides, platycodin D (glucose unit at C-3) is a major component and it has several pharmacological activities. Because of the high demand for this compound, we attempted to enzymatically covert platycodin D3 and platycoside E, having two and three glucose units at C-3, respectively, into platycodinD. In this study, we tested the ability of several glycosidases to transform platycosides, or morespecifically, the ability to transform platycoside E and platycodin D3 into platycodin D. To get pure platycodin D on a preparative scale, high-speed counter-current chromatography (HSCCC) with a solvent system ethyl acetae-n-butanol-water (1.2:1:2, v/v/v) was used for separation of the enzymatically-transformed product from the reaction mixture. 40.5 mg of platycodin D (99.8% purity) was obtained from 200 mg of the productin one-step seapartion.

저자정보

  • In Jin Ha Natural Products Research Institute, CollegeofPharmacy, Seoul NationalUniversity
  • Young Wan Ha Natural Products Research Institute, CollegeofPharmacy, Seoul NationalUniversity, Life Sciences Division, Korea Institute of Science and Technology, Seoul136-791,Korea
  • Minseok Kang Natural Products Research Institute, CollegeofPharmacy, Seoul NationalUniversity
  • Jongsung Lee Natural Products Research Institute, CollegeofPharmacy, Seoul NationalUniversity, Biospectrum Life Science Institute, GunpoCity 435-776, Korea
  • Deokhoon Park Biospectrum Life Science Institute, GunpoCity 435-776, Korea
  • Yeong Shik Kim Natural Products Research Institute, CollegeofPharmacy, Seoul NationalUniversity

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