원문정보
초록
영어
Ganglioside are ubiquitous membrane component in mammalian cells and suggested to play important roles in various cell functions such as cell-cell recognition, differentiation and transmembrane signaling. These compounds are localized in a glycosphingolipid-enriched microdomain on the cell surface and regulated by the glycosphingolipid composition. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, differential expression patterens of gangliosides between human vein endothelial cells (ECV304) and micro-pig aortic endothelial cells (MPAECs, primary cultured) was investigated. we examined expression level of high-performance thin-layer chromatography (HPTLC) showed that ECV304 cells contained GM3 and GM2 as major gangliosides. However, MPAECs contained only GM3 as major ganglioside. Furthermore, we confirmed that human serum induces cell death of MPAECs. As a results suggest that differential expression patterens of gangliosides of ECV304, MPAECs are related immune rejection response and cell death with xenogeneic serum in xenotransplantation by biomarker. This study was supported by a grant from the Korean Rural Development Administration Agenda Program, 2010030106112200103) and a research grant from the Ministry of Education, Science and Technology (KGC5401011), Republic of Korea.