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영어

Flavonoids are important natural pharmaceutical compounds found in almost all plants showing antioxidant, anti-inflammatory, antifungal, and anti-carcinogenic properties. Biosynthesis of glycosylated natural compound results in the formation of an overwhelming number of natural glycosides with numerous applications like solubility, stability, and bioavailability. To produce glycosylated flavonoids, the engineered E.coli cells were fed with Quercetin and Keampferol, the flavonol(or3-hydroxyflavone) exogenously and product was analyzed to be 3-O-rhamnosyl-quercetin and 3-O-rhamnosyl-kaempferol. Hence, based on the above strategy, the same engineered E.coli BL21(DE3, △pgi) cells generated by deleting the glucose-phosphate isomerase(Pgi) gene and heterologously expressing TDP-glucosesynthase, TDP-glucose4,6-dehydratase, TDP-4-keto-6-deoxyglucose3,5-epimerase, TDP-glucose 4-ketoreductase and arGt-3 (3-O-glycosyltransferase) from various sources were used as whole cell biotransformation reaction by feeding with fisetin exogeneously. The product was isolated and analyzed to be glycosylated fisetin by HPLC, ESI-MS and LC/MS, ESI-MS/MS. Since the glycosylated flavonoids have shown enormous pharmaceutical properties, the recombinant host generated can be utilize to discover glycosylated flvonoids as well as glycosylated quercetin and kaempferol as drugs.