earticle

영어

O-linked beta-N-acetylglucosamine (O-GlcNAc), one of the most important post-translational modification, regulates many cellular processes, including signaling, cell cycle, and transcription. However, the physiological function of O-GlcNAc in vivo is completely unknown. Some studies have shown that O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) modulates macronutrient storage and dauer formation in C. elegans and provide a genetic model for examining the role of O-GlcNAc in cellular signaling and insulin resistance. Here, we show that Drosophila melanogaster OGT and OGA regulates developmental growth through Insulin-IGF signaling pathway. Because not only human nucleocytoplasmic O-GlcNAc trasnferase (ncOGT) and OGT of drosophila melanogaster have highly conserved amino acid sequence but also human OGA and the hyaluronidase domain of drosophila OGA share the highly conserved sequence, our study provides insight into the mechanisms of O-GlcNAc modification in developmental growth and into the pathobiological basis for diabetes and obesity.