earticle

영어

Carbohydrate-protein interactions are involved in various biological events, including viral and bacterial infection, immune response, differentiation and development, the progression of tumor cell metastasis. Carbohydrate microarrays are new technology that has potentiated the high-throughput analysis of carbohydrate-protein interaction and contributes to significant advances of glycomics. Here, we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrates by reductive amination, in which reducing sugar groups were coupled with the linker. The modified carbohydrates were covalently attached to an amine-reactive NHS-activated glass surface by formation of stable amide bonds. This method was applied for construction of functional carbohydrate microarray platform to analyze carbohydrate-protein interactions. This array by using our method can be powerful tool for screening the specificity of GBPs (glycan-binding proteins) such as lectins, growth factors, antibody, and microbial toxins, and identification of carbohydrate-protein interactions.