earticle

영어

α-Glucosidase of Thermotoga maritima belongs to family 4 of the Glycosyl hydrolases. The enzyme α-glucosidase has the unusual property of requiring NAD+, divalent metal cations Mn2+ and reducing conditions (DTT) for activity. The gene aglS encoding 459 amino acid with 51 kDa was expressed under the control of the T7 promoter in Escherichia coli. It was found soluble form and thermo-stable up to 70oC. It showed hydrolyzing activity towards para-nitrophenyl-α-D-glucoside (pNP-α-Glc). The transglycosylation activity was tested using maltoseand sucrose as donor and cyclodextrin as acceptor. The molecular mass of cyclodextrin containing two glycosyl moieties is seen in ESI/MS when maltose and sucrose was used as donor. The further analysis is yet to be confirmed.