원문정보
초록
영어
Novel cDNAs of zOGT was identified in zebrafish (Danio rerio) genome and six different isoforms of transcriptional variants, designated var1 to var6 were isolated. Transcriptional variants of zOGT were differentially generated by alternative splicing and in particular, var1 and var2 were contained by a new exon-sequence, demonstrating that this form of zOGT was not found in mammals. We found that var1 and var2 were highly expressed at early phase including unfertilized egg until at 5-6 hpf whereas var3 and var4 were begins to be expressed at 5-6 hpf until late phase during embryogenesis. These results strongly indicate that var1 and va 2 are maternal transcripts. Coexpressionassay in E. coli–p62 system showed that zOGT catalytic activity of var3 and var4 was found to be active whereas those of other variants were inactive.