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Poster-21

N-Acetylglucosaminyltransferase I (GnTI) is Developmentally essential in Rice (Oryza sativa)

초록

영어

N-acetylglucosaminyltransferase I (GnTI) is an enzyme that controls the formation of hybrid and complex type N-glycans. Plant produce complex N-glycans with β1,2-xylose and core α1,3-fucose residues linked to the conserved core oligosaccharide. Production of heterologous glycoprotein in plant for therapeutic purposes is limited by the presence of plant specific oligosaccharide residues that are considered to arouse immunogenic response in the recipient body. Therefore, efforts for developing humanized N-glycosylation system in plants are required for the large scale production of safe therapeutic glycoproteins. In this regard, we isolated a rice mutant lacking GnTI activity. Biochemical analyses showed that rice gnt1 mutant predominantly produced high mannose type glycans and β1,2-xylose and core α1,3-fucose were absent on the endogenous glycoproteins. However, the rice gnt1 mutant showed severe developmental defects such as retarded shoot and root development, failure in tiller formation, and finally resulted in early developmental lethality. Interestingly, callus induced from gnt1 seeds was maintainable and proliferation rate of gnt1 callus was similar with that of WT. However, gnt1 calli were relatively smaller than that of WT. [Supported by EB-NCRC & BK21 program]

저자정보

  • Wahyu Indra Fanata Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Jeong Chan Moon Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Joo Mi Jeon Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Bo Hwa Son Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • In Jung Jung Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Jae Yong Yoo Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Jae Ho Cha Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Je Hein Kim Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Rikno Harmoko Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Ki Seong Ko Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Sang Yeol Lee Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University
  • Kyun Oh Lee Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University

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