earticle

영어

Plant-based expression of recombinant proteins offers significant advantages in terms of the production of pharmaceuticals for human diseases. These include cost-effective production of recombinant proteins due to the ease of scale-up, minimal possibility of product contamination by animal pathogens and the availability of natural storage organs such as tubers, fruits and seeds. Among the many plant-based production systems that have been developed for pharmaceuticals proteins, seeds have the useful advantage of accumulating proteins in a relatively small volume and in stable environment in which they are protected from degradation. In this respect, we overexpressed human glucocerebrosidase (GC) in Arabidopsis seeds driven by cruciferin 3 promoter. In attempt to produce mannose-terminated N-glycan of GC, the construct was introduced into Arabidopsis gnt1 mutant deficient in the activity of N-acetylglucosaminyltransferase I, the first enzyme in the complex glycan biosynthesis. Our results indicate that the human GC is highly expressed in the seed. [Supported by EB-NCRC & BK21 program]