earticle

영어

Accumulation of unfolded proteins in the lumen of endoplasmic reticulum (ER) induces ER stress and activates signaling pathway called unfolded protein response (UPR) for relief of ER stress. In yeast and mammalian cells, an ER-located transmembrane receptor protein kinase/ribonuclease called Ire1 transmits the signal to the nucleus culminating in the transcriptional activation of genes encoding an adaptive response. Plants including Arabidopsis are also containing homologous genes. However, it has been unclear whether the homologous genes are involved in similar mechanism in plants. Arabidopsis ire1 mutant did not show different phenotype compared to WT in normal and stress conditions. However, induction of ER chaperone genes including Bip3 were significantly delayed in ire1 compared to WT. To further investigate the role of AtIre1 in plant UPR signaling, we overexpressed AtIre1 under the control of CaMV 35S promoter. The result indicate that AtIre1 is possibly involved in UPR signaling in plants. [Supported by EB-NCRC & BK21 program]