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Poster-12

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

초록

영어

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

저자정보

  • Da-Young Park Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Yangkang So Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Kyung-Jin Lee Korean Research Institute of Bioscience & Biotechnology, Daejeon
  • Zhe Lu Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Eun-Yi Oh Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Kwang-Wok Min Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Seul-Ki Lee Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Jung-Hwan Lee Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Mi-Hyun Ahn Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Mira Song Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Doo-Byoung Oh Korean Research Institute of Bioscience & Biotechnology, Daejeon
  • Youngkwan Kim Department of Biological Science, College of Natural Sciences, Wonkwang University
  • Kisung Ko Department of Biological Science, College of Natural Sciences, Wonkwang University

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