earticle

영어

This research describes a simple method for direct detection of glycoproteins after affinity-capturing (or enrichment) on the plate using a combination of self-assembled monolayers (SAMs) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI). The terminal hydroxyl groups of the SAMs were activated by N,N‘-disuccinimidyl carbonate (DSC), followed by conjugation with aminophenylboronic acid (APB). The formed APB-functionalized self-assembled monolayers for MALDI (SAMDI) plates were successfully applied to the affinity-capturing of a glycoprotein from the mixture containing non-glycoproteins. The method developed in this work would provide an efficient and convenient analysis approach to proteomics.