earticle

영어

The aim of the present study was to analyze the influence of oxidative stress using sodium orthovanadate (SOV) on growth and production of water-soluble exo-polysaccharides in the photosynthetic green alga Haematococcus lacustris. Fourier Transform Infrared (FT-IR) analysis and elemental analysis were performed to determine the primary chemical structure of the polysaccharide, and its monosaccharide composition was characterized by a High Performance Anion-Exchange Chromatography (HPAEC) using Bio-LC (DX-500 Chromatography System, Dionex Co., USA). The molecular weight of the compound was determined by size-fractionation HPLC. Higher production of extracellular polysaccharide from H. lacustris following treatment of SOV was observed at day 2 as compared to untreated controls. Consequently, the total content of crude carbohydrates produced from SOV treated cells (51.2 mg/ml) was approximately 1.7- fold higher than that of the control cells (31.1 mg /ml) at day 2. The molecular mass of the major component of the purified water-soluble polysaccharide was estimated to be approximately 135-kDa by size-fractionation HPLC. In addition, Fourier Transform Infrared (FT-IR) and elemental analysis demonstrated the presence of sulfate groups in this polysaccharide. Taken collectively, our data suggest that oxidative stress by SOV to the cells inhibits cell growth and promotes the expression of water-soluble sulfated polysaccharides from H. lacustris.