earticle

영어

Heterologous expression of different combinations of genes from the 4,6-disubstituted aminoglycoside biosynthetic gene cluster in a non-aminoglycoside producing strain of S. venezuelae caused production of intermediates from the biosynthetic pathway. This provided experimental evidence for assigning the functions of individual gene products and also allowed us to recognize a complete biosynthetic pathway for generation of 4,6-disubstituted aminoglycosides. This work has established an efficient heterologous expression platform based on a genetically amenable streptomycete to dissect the biosynthetic pathways of other aminoglycoside antibiotics produced by less tractable actinomycete.