earticle

영어

Cancer is often difficult to early diagnose, but early diagnosis is a crucial factor for good outcome in the cancer. N-acetylglucosaminyltransferase V catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in the pathogenecity of colon cancer cell. One of the related example is an aberrant TIMP-1-mediated cancer progression in which deterioration of the quality of TIMP-1 is induced by glycosyl alterations through GnT-V catalysis, leading to mitigated MMP inhibition responsible for an strengthened invasive/metastatic potential of cancer cells. Accordingly the identification of target proteins for GnT-V has been an important subject for cancer biomarker discovery. We developed a protocol in which L-PHA, a lectin recognizing b1,6-GlcNAc, is conjugated to avidin-agarose complex to capture b1,6-GlcNAc-carrying serological glycoproteins and the captured glycoproteins were identified using an LTQ-FTICR mass spectrometer. Candidate proteins showing differential amounts between normal and cancer sera were confirmed by western blot analysis and will be subject to validation for valid biomarker for colon cancer.