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Poster-34

O-GlcNAc Modification in various cellular events

초록

영어

β-O-linkedN-acetylglucosamine(O-GlcNAc) is a nucleocytosolic post-translational modification on serine and threonine residues that is dynamically regulated by O-GlcNActransferase and O-GlcNAcase. Many proteins are O-GlcNAcylated in response to various cellular processes, including transcription, proliferation, apoptosis and signal transduction. So, we have studied the function of O-GlcNAc modification on several proteins. We found that O-GlcNAcylated NF-kB was translocated into nucleus and had increased transcriptional activity. We report increased O-GlcNAcylation in non-small cell lung carcinoma A549 cells and various other cells in response to glucose deprivation. Also we show that glycogen degradation in response to glucose deprivation provides a source for UDP-GlcNAc required for increased O-GlcNAcylation under this condition. we demonstrate that Snail is O-GlcNAcylated and adjacent-site occupancy inhibits phosphorylation by GSK-3, resulting in increased Snail stability and attenuation of E-cadherin proximal promoter activity and transcription level. Furthermore, Overexpression of OGT induces in vivo invasion program of epithelial cancer cells by Snail-dependent manner. O-GlcNAc modification seems to be involved in neurite outgrowth in cultured neuronal cells. O-GlcNAcylation is highly conserved, so we were able to find several novel proteins modified with O-GlcNAc in Drosophila S2 cell line. Also we found O-GlcNAcylated site in ncOGT outside of TPR by using Q-TOF MS. Now we focus on the activities and function of ncOGT by mutagenesis studies. As stated above, O-GlcNAcylation is related to many cellular metabolism. And we already have found the function of O-GlcNAc modification on several important proteins. We'll keep on concentrating on this interesting post-translational modification. If you have any interest, feel free to contact us.

저자정보

  • Jeong Gu Kang Department of Biology, Yonsei University
  • Sang Yoon Park Department of Biology, Yonsei University
  • Suena Ji Department of Biology, Yonsei University
  • Sujin Park Department of Biology, Yonsei University
  • Insook Jang Department of Biology, Yonsei University
  • Hyeon Gyu Seo Department of Biology, Yonsei University
  • Yeon Jung Kim Department of Biology, Yonsei University
  • Jin Won Cho Department of Biology, Yonsei University

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