earticle

영어

Recent studies demonstrated that human mesenchymal stem cells (hMSCs) share molecular and cellular characteristics with bone marrow-derived mesenchymal stem cells (MSCs). hMSCs have the potential to differentiate into neural cells. Gangliosides are complex glycophingolipids with one or more sialic acids, the major components of cytoplasmic cell membranes. Numerous studies confirmed that the types of gangliosides and their expression levels are developmentally controlled and cell type-specific. Our recent studies proposed that expression of gangliosides is closely related to neural differentiation of embryonic stem cells in vitro. In this study, we investigated whether hMSCs are capable of differentiating into neural cells, and we analyzed the role of gangliosides in the neuronal differentiation of hMSCs. FACs analysis showed that the established cells have MSC characteristics. The cells did expressed MSC-specific surface antigene (CD44 and CD105), but not the hematopoietic markers CD45 and CD117. Next, hMSCs were differentiated into neuronal cell with induction media for 30h. Neuronal induced cells maintenance with differentiation media for 2weeks. High-performance thin-layer chromatography (HPTLC) showed that gangliosides GM3, GD3 and GD1a expressed in differentiated into neuronal cells for 2weeks, especially GD3 increased comparison to control cell. Immunofluorescence staining also agreed with the results of HPTLC analysis. These differentially expressed gangliosides suggest that gangliosides may have specific functions in stem cell and during neuronal differentiation. Therefore, thess results also suggest that regulation of gangliosides expression have used as differentiation marker of neuronal cell from hMSCs.