earticle

영어

Ganglioside are ubiquitous membrane component in mammalian cells and suggested to play important roles in various cell functions such as cell-cell recognition, differentiation and transmembrane signaling. These compounds are localized in a glycosphingolipid-enriched microdomain on the cell surface and regulated by the glycosphingolipid composition. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, we differential expression patterens of gangliosides between HEK293 cells (human embryonic kidney cells) and mini-pig kidney cells (NIH-mini pig, primary cultured) was investigated. we examined expression level of high-performance thin-layer chromatography (HPTLC) showed that HEK293 cells and mini-pig kidney cells contained GM3, GM2 and GD3 as major gangliosides. Moreover, ganglioside GM3, GM2, GM1 and GD3 which are gangliosides of mini-pig kidney cells expressed more than HEK293 cells. Especially, ganglioside GT1b expressed in HEK293 cells, not in mini-pig kidney cells. and GM1 expressed in mini-pig kidney cells, not in HEK293 cells. As a results suggest that differential expression patterens of gangliosides of HEK293, mini-pig kidney cells are related immune rejection response in xenotransplantation by biomaker.