원문정보
초록
영어
Ganglioside GQ1b has important functions in neuronal differentiation and neuronal development processs. The regulation of ganglioside levels is relationship to the induction of neuronal cell differentiation. In the previous study, we showed that expression of GT1b and GQ1b gangliosides in undifferentiated mouse embryonic stem cell were exhibit during their neural differentiation. We evaluated the co-localization of ganglioside GQ1b and neuroanl markers in an in vitor model of neuronal differentiation in mouse embryonic stem and found that neuronal differentiation was facilitated by the presence of increased gangliosides, especially GQ1b, due to daunorubicin. Furthermore, we investigated that important effect of GQ1b by overexpression and konckdown GQ1b in the neural differentiate. First, we designed a primer of a2,8-sialyltransferase (ST8Sia V) for producing GQ1b. In this study, we inserted ST8Sia V gene to overexpression (pEGFP-C2), and knock-down (pLKO.1 puro ShRNA) vectors. We will confirm the efficient transfectionof mouse embryonic stem cell by green fluorescent protein (GFP) and expressin of ganglioside GQ1b by immunofluorescence staining analyses.