earticle

영어

Thermus caldophilus GK24 (TCA) is an extremothermophile growing at 70℃ ,providing industrially important enzymes. From TCA genome sequence, all of gene sresponsible for carbohydrate biosynthetic network were identified .Among them, 120 enzyme genes have been expressed in E. coli and their reactions were characterized. In this lecture, Thermus-derived Carbohydrate Biosynthetic Network’ will be introduced, particularly focused on in vitro operation of a-glucan network’, glycolysis and pentose-phosphate pathway. 1) ‘a-Glucan Network’ showed to be the practical synthetic methods of various sugars such as trehalose, fructose, phosphosugars, sugar-nucleotides, a-glucan and so on. 2) in vitro Glycolysis using glycolytic enzymes have been operated for synthesizing pyruvate from glucose or glucose-1-phosphate. Accordingly, we could have managed to operate the glycolysis in flask. 3) And one pot reaction containing a-glucan phosphorylase, phospho glucomutase, glucose-6-phosphate isomerase, transaldolase and transketolase could synthesize sedoheptulose-7-phosphate from starch. Overall, ‘in vitro Cell Factory’ utilizing thermostable carbohydrate enzymes turned out to be a practical synthetic model to produce C3-C9 sugars, phosphosugars, sugar-nucleotides and biopolymers.