earticle

영어

The crushed fruiting body of Alaskan Phellinus pini (Brot.) Murrillwas extracted in boiling water for 4 h with the yield of 20.5% in dry mass. From which the ethanol precipitate (EP) and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 14.2 % in dry mass, respectively. From the EP preparation, two major carbohydrate-positive fractions, named EP-AV1 and EP-AV2, were purified by mainly Sepharose CL-4B column. Both EP-AV1 and EP-AV2 inhibited the plaque formation by CVB3 on HeLa cells by 32% and 84%, respectively, at 1 mg/mL, with EC50 of over 1 mg/ml(EP-AV1) and 0.576 mg/ml (EP-AV2), and the antiviral activity of these two polysaccharides against HSV-1 in Vero cells, inhibited in a dose-dependent manner at much lower concentrations (1 to 50 μg/ml), with EC50 of 0.20 μg/ml (EP-AV1) and 0.21 μg/ml (EP-AV2), respectively. Also, EP-AV1 and EP-AV2 inhibited neuraminidase activity by 27.4% and 52.4% at 1.7 mg/ml, and 44.1% and 73% at 3.4 mg/ml, respectively. EP-AV1 was shown to be a heteropolysaccharide containing glucose as the main sugar residue with mole percentage of 53.4% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%). EP-AV2 was also a heteropolysaccharide with glucose as the main sugar (56.1%) and other minor sugars similar to that of EP-AV1 but relatively lower proportion of galactose (10.3%). The HPAEC-PAD analysis after laminarinase digestion showed that both polysaccharides contain β-1,3-linked glucose residues, suggesting that these polysaccharides are a β-(1,3)-glucan. The results of this study suggest that these water-soluble polysaccharides of Alaskan P. pini fruiting body can be a strong candidate for the development of a potent broad-range antiviral agent.