원문정보
초록
영어
Sialic acid containing structures in eukaryotic systems play important roles in a variety of physiological and pathological processes, including cell–cell interactions, inflammation, fertilization, viral infection, differentiation, malignancies, and cell signaling etc. Especially sialic acid derivatives product in living system are spotlighted because sialic acid is an intermediate to inhibit viral disease like SARS, bird flu infection is reported. Although, sialytransferase has been expressed in E.coli, because of its toxicity many problems arise in use to animal or human. In this study, to express sialytransferase gene from Pasteurella multocida in yeast, a non-toxic widely used strain. Cloning was carried out in yeast shuttle vector pYES2 and expressed. Enzymatic activity was analyzed by TLC and Bio-LC.