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논문검색

Poster-1

N-glycosylation Pathway Engineering in Insect Drosophila S2 Cells Using Recombinant Baculoviral Multiple Protein Expression Platform

원문정보

Kyoung Ro Kim, Dong Gyun Kang, Yeon Kyu Kim, So Young Jang, Young Hwan Kim, Hyung Joon Cha

한국당과학회 한국당과학회 학술대회 2009 한국 당과학회 하계 학술대회 2009.06 pp.23-24
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Insect cell lines have been used extensively for the production of numerous functional eukaryotic proteins. However, insect cell systems have limited commercial use due to a lack of complex glycosylation. Glycosylation is very crucial factor for use of pharmaceutical proteins. While, baculovirus is used as a strong gene delivery carrier for transfection of insect cells. Especially, Drosophila S2 cells have been developed as a plasmid-based and non-lytic expression system using recombinant baculovirus, infects into insect cells and does not require any selection markers. A gene encoding human erythropoietin (hEPO) or human 90K (h90K) fused with a hexa- histidine (His6) tag under the control of the Drosophila originated metallothionein promoter (pMT) was stably transfected into Drosophila S2 cells. In the preliminary studies, we found that expressed proteins had simple N-glycan structures instead of complex structures. We thought that this result caused by N-acetylglucosaminidase (GlcNAcase) through enzyme assay experiment. Therefore, this research will propose that suppression of GlcNAcase and several expression of N-glycosyltransferases using recombinant baculovirus- based multiple protein expression platform.

저자정보

  • Kyoung Ro Kim National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang
  • Dong Gyun Kang National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang
  • Yeon Kyu Kim National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang
  • So Young Jang Mass Spectrometry Research Team, Korea Basic Science Institute, Ochang
  • Young Hwan Kim Mass Spectrometry Research Team, Korea Basic Science Institute, Ochang
  • Hyung Joon Cha National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang

참고문헌

자료제공 : 네이버학술정보

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