earticle

영어

The crushed fruiting body of Alaskan Phellinus pini (Brot. ex Fr.) Ames was extracted in boiling water for 4 h with the yield of 20.5% in dry mass. From which the ethanol precipitate (EP) and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 14.2% in dry mass, respectively. Whereas ES did not show any detectable level of antiviral activity, EP showed significant dose-dependent inhibition of plaque formation by coxackievirus B3 (CVB3) on HeLa cells, with an EC50 (50% effective concentration) of 0.45 mg/mL, and strongly inhibited plaque formation up to 94.9% against herpes simplex virus (HSV-1) on Vero cells at 5 μg/mL. EP also effectively inhibited neuraminidase activity in a dose-dependent manner, showing up to 80% inhibition at 1.7 mg/mL. From the EP preparation, two major carbohydrate-positive fractions, named EP-AV1 and EP-AV2, were purified by mainly Sepharose CL-4B column chromatography with apparent molecular mass of approximately 1,006 kDa and 100 kDa, respectively. Both EP-AV1 and EP-AV2 inhibited the plaque formation by CVB3 on HeLa cells by 32% and 84%, respectively, at 1 mg/mL. EP-AV1 was shown to be a heteropolysaccharide containing glucose as the main sugar residue with mole percentage of 53.4% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%). EP-AV2 was also a heteropolysaccharide with glucose as the main sugar (56.1%) and other minor sugars similar to that of EP-AV1 but relatively lower proportion of galactose (10.3%). The TLC analysis after laminarinase digestion showed that both polysaccharides contain, at least partly, β-1,3-linked glucose residues and β-1,6-linked glucose residues, suggesting that these polysaccharides are a β-(1,3)(1,6)-glucan.