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영어
Aminoglycosides are a group of antibiotics that are used to treat certain bacterial infections. This group of antibiotics includes at least eight drugs, amikacin, gentamicin, kanamycin, neomycin, netilmicin, paromomycin, streptomycin, and tobramycin. One of them, we study the biosynthesis of UDP-kanosamine. UDP-kanosamine(UDP-3-amino-3-deoxyglucose) is a sugar moiety of kanamycin that was highly inhibitory to growth of plant-pathogenic oomycetes and moderately inhibitory to certain fungi and inhibited few bacterial species tested. Biosynthesis of UDP-kanosamine starts with UDP-glucose via UDP-3-keto-glucose. KanC converts UDP-glucose to UDP-3-keto-glucose by dehydrogenation and KanD converts UDP-3-keto-glucose to UDP-kanosamine by aminotransferation. Here we have cloned KanC and KanD in expression vector pET32a+ and expression was carried out. The soluble protein of KanC and KanD were used for enzymatic assay to synthesize UDP-kanosamine and further analysis was carried out.