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Poster-17

Metabolic engineering of E. coli for the production of glycosylated flavonoids

원문정보

Dinesh Simkhada, EuiMin Kim, Nagendra Prasad Kurumbang, Tae-Jin Oh, Hei Chan Lee, Jae Kyung Sohng

한국당과학회 한국당과학회 학술대회 2008 Eastern Asian Glycoscience Symposium 2008.11 pp.64-64
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Glycosylation of flavonoid play crucial roles in stabilization of antocyanins and cyanidins; storage of flavonoid and terpenoids; and regulation of hormones. In addition, glycosylation has been recognized as one of the important mechanisms for detoxification of exogenous compounds. Here in this research, we have metabolically engineered the E. coli BL21DE3 (Δ pgi mutant) host to generate four different engineered host to produce glycosylated flavonoid. E. coli BL21DE3 (Δ pgi mutant) was engineered by integration of GalU, expression of CalS8 (dehydrogenase) and CalS9 (decarboxylase) together by cloning in pDuet/ampr vector and expression of four different 3-O-glycosyltransferase and 7-O- glycosyltransferase gene from Arabidopsis thaliana. Engineered hosts are expected to produce glucosyl as well as xylosyl glycosylated flavonoids which are characterized by HPLC as well as LC-MS analysis.

키워드

저자정보

  • Dinesh Simkhada Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University
  • Jae Kyung Sohng Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University
  • EuiMin Kim Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University
  • Nagendra Prasad Kurumbang Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University
  • Tae-Jin Oh Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University
  • Hei Chan Lee Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical, Sun Moon University

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자료제공 : 네이버학술정보

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