원문정보
초록
영어
Sialylated sugar chains are present at the cell surface of various animal species. Due to their position, they are thought to serve important roles in a large variety of biological functions such as cell–cell and cell–substrate interactions, bacterial and virus adhesion, and protein targeting. We present a bio-conversion process for the conversion of lactose, N-acetyl- glucosamine and CMP into 2,3-sialyllactose with six enzymes. This process consists of two steps; the first step is from N-acetyl-glucosamine and CMP to CMP-neuraminic acid with five enzymes (GlcNAc-2-epimerase, NeuAc aldolase, CMP kinase, acetate kinase and CMP-NeuAc synthetase) including ATP recycle system to yield over 95% by one-pot reaction and the second step is from lactose and CMP-neuraminic acid with sialyltransferase to yield over 90%. The second step can apply to synthesize the various sialyllactose (sialyloligosaccharide) depend on acceptors (lactose and N- acetyl-lactosamine) and sialyltransferase (2,3-sialyltransferase or 2,6- sialyltransferase).