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영어

The α-viniferin was reported to show anti-inflammatory effects. Cyclooxygenase-2 (COX-2) expression was detected in most adenomas and aberrant crypt foci (ACF). Glycosphingolipids are involved which cell proliferation, differentiation and cellular interactions in the plasma membrane remain to be established. In the present study, experiments were designed to assess the potentialchemopreventive properties of α- viniferin on azoxymethane (AOM)-induced colorectal carcinogenesis. Administration of α-viniferin at dose 0.05 and 0.1mg/mouse during AOM-treatment for 5 weeks resulted in a dose-dependent reduction of ACF formation, being 75% and 64% (P<0.05) inhibition of the control value. Treatment of α-viniferin at dose 0.05 and 0.1mg/mouse for 28 weeks resulted in a dose-dependent suppress of colorectal carcinogenesis, being 80.2% and 49% (P<0.05) inhibition of the control. COX-2 expression were decreased on ACF tissue and colorectal tumor tissue treated with α-viniferin. In vitro, we demonstrated that α-viniferin play the roles such as activity of apoptosis, increase of caspase-3 and inhibition of COX-2 expression in Caco-2 colon cancer cells. High -performance thin-layer chromatography (HPTLC) shows gangliosides GM3, GM2 and GT1b increasein the Caco-2 cells are treated with α-viniferin. Immunofluorescence stain results show GT1b and GM3 expression significantly increase in theCaco-2 cells by α-viniferin. The results indicated that suppression of colorectal carcinogenesis was related to the activityof apoptosis through caspases-3 activity, COX-2 inhibition and gangliosides expression by α-viniferin. These results suggested that α-viniferin may play roles of chemopreventiveproperty in carcinogens-induced colorectal carcinogenesis of mice.