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Purpose: This study was to investigate oral hygiene knowledge and the actual condition of oral care for the students in the public health and non-public health Departments. Methods: The survey sample consisted of 262 randomly selected students. 197 of them are public health-related students and 65 are non-public health-related students. The questionnaires used in this study consisted of 4 items for general information, oral health status of 2 items, 5 items for Oral hygiene knowledge and actual condition of oral care of 5 items. Collected data were analyzed by Frequency Analysis t-test and Crosstabs using SPSS(Statistical Package for Social Sciences) Win 19.0 statistics program. Results: Regarding general characteristics of the subjects, there were 53.8% of first-year student, 26.0% of secondyear student and 20.2% of third-year student among 52.3% of males and 47.7% of females. The subjects received less than “100,000” won(13.0%), “100,000-190,000” won (5.0%), “200,000-290,000” won(30.9%), “300,000-390,000” won (33.2%) and more than “400,000” won(17.9%) a month for an allowance. The subjects had ‘none’ (44.3%), ‘1’(6.5%), ‘2’(12.6%), ‘3’(5.0%), ‘4’(5.3%), ‘more than’ 5(0.8%) of dental caries and ‘do not know’ were 25.6%. The numbers of dental prosthetics were ‘no’ (35.5%), ‘1’(13.7%), ‘2’(17.2%), ‘3’(10.3%), ‘4’(11.5%), and ‘more than 5’(11.8%). The public health students(84.8%) had a higher positive response rate than the non-public health students(66.2%). The public health students(80.2%) had a higher negative response rate than the non-public health students(78.5%) in the result of onset of gum disease pain awareness. The non- public health students(65.5%) had a higher negative response rate than the public health students(68.3%)) in the result of heredity of gum disease. The t-test showed that public health-related department students(M=8.264, SD=0.821) had more knowledge about oral hygiene than non-public health-related department students(M=8.015, SD=1.082). ‘do not use’ in the oral care products except toothpaste and toothbrush showed that public health-related department students(60.4%) use the products more than non-public health- related department students(66.2%). Conclusion: In this study, public health- related department students had higher oral hygiene knowledge and actual condition of oral care compared to non-public health-related students, but low in practical action. Due to the lower level of dental care products use in non-public health-related department, a continuous oral care education program is required.