earticle

영어

We evaluated the components present in human sweat by headspace solid phase microextraction (HSPME) and 2nd dimensional gas chromatography (GC×GC) - time of flight mass spectrometry (TOFMS). We collected sweat sample from 3 male donor. A total of 269 compounds were identified as components of human sweat. Compound classes present in human sweat, such as Carboxylic acids, Alcohols, Aldehydes, Aliphatics/Aromatics, Amides/Amines, Esters, Halides, Heterocyclics, ketones, Thio/Thioesters/sulfonyls, Oxide, Sulfides, Nitro compouds et al. We found individually distinct components as 2-nonenal, 2-undecenal with age and identified the influence of outside sources (soaps, lotions, perfumes) as Lily aldehyde, 2,6-dimethylnaphthane, etc and environmental factors(dibenzo thiophene, 9H-fluoren-9-one, fluorene, fluoranthene, 9H- Xanthene, 1H-Indene). The results suggest that the analysis based on individual characteristics of human sweat emanations could provide useful information for the forensic science investigation.

한국어

본 연구는 한국인 남성 50, 29, 24 연령 3인의 땀을 시료로 하여 2nd dimensional gas chromatography(GC×GC) - time of flight mass spectrometry (TOFMS)와 headspace solid-phase microextraction (HSPME)을 응용하여 땀의 성분분석에 적절한 방법을 조사하고 개체별 특징을 파악할 수 있는 자료를 얻고자 하였다. 땀에서 검출된 성분으로는 Carboxylic acids, Alcohols, Aldehydes, Aliphatics/Aromatics, Amides/Amines, Esters, Halides, Heterocyclics, ketones, Thio/Thioesters/sulfonyls, Oxide, Sulfides, Nitro compouds등 총 269종이 검출되는 것을 확인하였으며 3인에게서 확인되는 공통검출성분으로는 Alcohol류의 2-decen-1-ol, 2-phenoxy ethanol를 비롯하여 Aldehydes류 nonanal, dodecanal, octanal과 Aliphatics/Aromatics의 tetradecane, nonadecane과 2,6-dimethyl-naphthalene, Esters류에는 4-methyl-benzoic acid, oxalic acid, 그외에 nitro화합물인 7-Acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, 2,4-bis(1,1-dimethyl ethyl)-phenol 등 25종이검출되는 것으로 확인되었다. 개인별 특징적인 성분으로는 연령의 증가에 따라 2-nonenal, 2-undecenal이 검출되는 것으로 확인되고 Lily aldehyde, 2,6-dimethyl-naphthane과 같은 세정용 비누류, 의복, 로션등과 같은 생활사용 오염과 dibenzo thiophene, 9H-fluoren-9-one, fluorene, fluoranthene, 9H-Xanthene, 1H-Indene과 같은 노출환경의 예측이 가능한 다양한 휘발성분이 검출됨을 확인하였다. 땀의 개체 특성분석으로 과학수사에의 활용이 기대된다.