원문정보
An Increased Intracellular Calcium Ion Concentration in Response to Dimethyl Sulfoxide Correlates with Enhanced Expression of Recombinant Human Cyclooxygenase 1 in Stably Transfected Drosophila melanogaster S2 Cells
초록
영어
Dimethyl sulfoxide (DMSO) increased the intracellular calcium ion concentration in stably transfected Drosophila melanogaster S2 cells expressing recombinant cyclooxygenase 1 (COX-1). DMSO did not increase the Drosophila NOS (dNOS) transcript level in calcium chelatortreated cells. Expression of recombinant COX-1 due to DMSO was diminished in cells treated with calcium chelators or channel blockers. Our results indicate that an increased intracellular calcium ion concentration due to DMSO is associated with up-regulation of the dNOS gene, leading to enhanced expression of COX-1.
목차
1. Introduction
2. Materials and Methods
2.1. Cell culture
2.2. Measurement of intracellular calcium ion concentration
2.3. SDS-PAGE and Western blot analysis
2.4. Northern blot analysis
3. Results and Discussion
3.1. Effect of DMSO on expression of recombinant COX-1 from stably transfected S2 cells
3.2. Effect of DMSO on intracellular calcium ion concentration
3.3. Effect of the intracellular calcium ion concentration on up-regulation of the dNOS transcript by DMSO
3.4. Effect of calcium chelators on expression of recombinant COX-1 in DMSO-treated stably transfected S2 cells
3.5. Effect of calcium channel blockers on expression of recombinant COX-1 in DMSO-treated stably transfected S2 cells
4. Conclusions
Acknowledgement
References