원문정보
Effects of Argon-plasma Jet on the Cytoskeleton of Fibroblasts : Implications of a New Approach for Cancer Therapy
초록
영어
Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.
목차
1. 서론
2. 재료 및 방법
2.1. 세포배양
2.2. 아르곤 플라즈마의 세포 처리
2.3. 핵 염색법
2.4. 형광염색법
2.5. 면역 블로팅 (Immunoblotting, IB)
3. 결과 및 고찰
3.1. 아르곤 플라즈마 처리에 의한 세포 표면 변화
3.2. 아르곤 플라즈마 처리에 의한 세포 내 골격구조의 변화
4. 결론
감사
References