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연구논문

레스베라트롤의 HT-29 대장암 세포증식 및 이동성 억제효과

원문정보

Effects of Resveratrol on Migration and Proliferation in HT-29 Colon Cancer Cells

이솔화, 박송이, 김인섭, 박옥진, 김영민

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초록

영어

Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 재료
  2.2. 세포 배양
  2.3. MTT assay에 의한 암세포의 생존율 측정
  2.4. Wound healing assay에 의한 HT-29 cell migration 관찰
  2.5. Western blotting
  2.6. 통계처리
 3. 결과 및 고찰
  3.1. 레스베라트롤이 HT-29 세포의 증식에 미치는 영향
  3.2. 레스베라트롤이 HT-29 세포의 이동성에 미치는 영향
  3.3. 레스베라트롤이 matrix metalloproteinase-9 (MMP-9)활성에 미치는 영향
  3.4. 레스베라트롤이 p-AMPK, COX-2, VASP 및 VEGF 발현에 미치는 효과
  3.5. AMPK, COX-2 저해제 처리가 p-AMPK, COX-2, VASP및 VEGF 신호경로에 미치는 영향
 4. 결론
 감사
 References

저자정보

  • 이솔화 Sol Hwa Lee. 한남대학교 생명나노과학대학 생명시스템과학과
  • 박송이 Song Yi Park. 한남대학교 생명나노과학대학 생명시스템과학과
  • 김인섭 In-Seop Kim. 한남대학교 생명나노과학대학 생명시스템과학과
  • 박옥진 Ock Jin Park. 한남대학교 생명나노과학대학 식품영양학과
  • 김영민 Young Min Kim. 한남대학교 생명나노과학대학 생명시스템과학과

참고문헌

자료제공 : 네이버학술정보

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