원문정보
Effects of Resveratrol on Migration and Proliferation in HT-29 Colon Cancer Cells
초록
영어
Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.
목차
1. 서론
2. 재료 및 방법
2.1. 재료
2.2. 세포 배양
2.3. MTT assay에 의한 암세포의 생존율 측정
2.4. Wound healing assay에 의한 HT-29 cell migration 관찰
2.5. Western blotting
2.6. 통계처리
3. 결과 및 고찰
3.1. 레스베라트롤이 HT-29 세포의 증식에 미치는 영향
3.2. 레스베라트롤이 HT-29 세포의 이동성에 미치는 영향
3.3. 레스베라트롤이 matrix metalloproteinase-9 (MMP-9)활성에 미치는 영향
3.4. 레스베라트롤이 p-AMPK, COX-2, VASP 및 VEGF 발현에 미치는 효과
3.5. AMPK, COX-2 저해제 처리가 p-AMPK, COX-2, VASP및 VEGF 신호경로에 미치는 영향
4. 결론
감사
References