원문정보
Developments of Culture Media for Human Skin Stem Cell, and Evaluation of Efficacy of Cosmetics Containing Culture Media
초록
영어
This research evaluates the efficacy of cosmetic products manufactured by applying stem cell culture techniques. The results are obtained after analysing the characteristics of cells cultured in the medium and evaluating function of cosmetics and the effectiveness using MTS procedure. When culturing human skin cell and skin stem cell after manufacturing culture medium by mixing approved cosmetics ingredients, the growth and proliferation of cells were assured. Also associated with skin regeneration from cultured cells and cell function was remarkably improved. With the culture medium of skin stem cell, Skin essence was manufactured. This cosmetics showed outstanding improvement effect on skin condition at sensory test of skin moisturizing, anti-wrinkle, whitening at the time of its use. In addition, a clinical test was conducted to assess the effectiveness of the Skin essence by applying skin essence containing culture medium after MTS procedure used as a physical stimulation method in efficient absorption and delivery of cosmetic ingredients. After MTS procedure, application of skin essence was represented to reduce erythema amount of facial skin and significantly increase moisture content. We conclude that cosmetics including culture medium ingredients of skin stem cell promotes the regeneration of skin whitening and anti-winkle and improves the status of trouble by soothing skin irritation by MTS procedure. Futhermore, this cosmetics showed the striking cosmetic effect of improving skin condition by maintaining and increasing skin moisture.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 화장품의 원료로 허용된 성분으로 줄기세포 배양 배지의 제조
2. 인간의 피부줄기세포 분리 및 성장속도측정
3. 배양된 세포의 피부줄기세포 표지인자 발현특성 분석
4. 화장품의 제조 및 유효성 평가
Ⅲ. 연구결과
Ⅳ. 고찰
Ⅴ. 결론
참고문헌
