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RESEARCH ARTICLE

인간 피부줄기세포 배지의 개발과 이를 조성물로 제조한 화장품의 유효성 평가

원문정보

Developments of Culture Media for Human Skin Stem Cell, and Evaluation of Efficacy of Cosmetics Containing Culture Media

최은주, 조홍범, 윤현수

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

This research evaluates the efficacy of cosmetic products manufactured by applying stem cell culture techniques. The results are obtained after analysing the characteristics of cells cultured in the medium and evaluating function of cosmetics and the effectiveness using MTS procedure. When culturing human skin cell and skin stem cell after manufacturing culture medium by mixing approved cosmetics ingredients, the growth and proliferation of cells were assured. Also associated with skin regeneration from cultured cells and cell function was remarkably improved. With the culture medium of skin stem cell, Skin essence was manufactured. This cosmetics showed outstanding improvement effect on skin condition at sensory test of skin moisturizing, anti-wrinkle, whitening at the time of its use. In addition, a clinical test was conducted to assess the effectiveness of the Skin essence by applying skin essence containing culture medium after MTS procedure used as a physical stimulation method in efficient absorption and delivery of cosmetic ingredients. After MTS procedure, application of skin essence was represented to reduce erythema amount of facial skin and significantly increase moisture content. We conclude that cosmetics including culture medium ingredients of skin stem cell promotes the regeneration of skin whitening and anti-winkle and improves the status of trouble by soothing skin irritation by MTS procedure. Futhermore, this cosmetics showed the striking cosmetic effect of improving skin condition by maintaining and increasing skin moisture.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 화장품의 원료로 허용된 성분으로 줄기세포 배양 배지의 제조
  2. 인간의 피부줄기세포 분리 및 성장속도측정
  3. 배양된 세포의 피부줄기세포 표지인자 발현특성 분석
  4. 화장품의 제조 및 유효성 평가
 Ⅲ. 연구결과
 Ⅳ. 고찰
 Ⅴ. 결론
 참고문헌

저자정보

  • 최은주 Eun Joo Choi. 서경대학교 미용예술학과, (주)에스미디아
  • 조홍범 Hong Bum Cho. 서경대학교 화학생명공학과
  • 윤현수 Hyun Soo Yoon. 서경대학교 화학생명공학과, (주)에스미디아

참고문헌

자료제공 : 네이버학술정보

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